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Most Common Component Of ELISA Kit

Monday , 8, March 2021 Comments Off on Most Common Component Of ELISA Kit

The most common components in an ELISA kit include a microtiter plate, sample diluent, control, standard or calibrator, conjugate, substrate, stop solution, wash buffer. If you are looking for elisa service then you may browse the internet.

Now let's take a closer look at the functions of these components:

Microtiter Plates: The solid phase typically has 96 wells and each well is not covalently coated with antigens or antibodies.

Sample Diluent: A solution whose sample is diluted to a concentration suitable for ELISA.

Control; There are usually positive and negative controls. The OD value of the negative control must not be higher than the standard value and the OD value of the positive control must not be lower than the standard value, otherwise, the test is invalid.

Standards or calibrators: Manufacturers offer different standards for known concentrates or the same concentrate (calibrator). Using a standard, the researcher can derive a curve and read the sample concentrate value from that curve. With the calibrator, the researcher can determine the boundary value and from this determine the ratio of the sample to the calibrator, the paternal value of the concentrate.

Conjugation: Contains secondary antibodies that bind to antibody-antigen complexes. And it binds to the enzyme to react with the substrate.

Substrate: The substrate reacts with enzymes and causes a color change, i.e. a measurement of the amount of antibody or antigen. TMB (3,3 ', 5,5'-tetramethylbenzidine) is a substrate commonly used in ELISA kits.

Stop the solution: Stop the reaction, usually a mixed solution of H2SO4 and HCl.

Wash Buffer: When the user removes antibodies or unbound antigens, select a suitable buffer to wash the concentrate.